quantitative bisulfite-pyrosequencing method Search Results


dna methylation analysis bisulfite/pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc dna methylation analysis bisulfite/pyrosequencing
Example of how some gene regions were chosen for examination in this study on the basis of available RRBS <t>DNA</t> <t>methylation</t> profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE
Dna Methylation Analysis Bisulfite/Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna methylation analysis bisulfite/pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
dna methylation analysis bisulfite/pyrosequencing - by Bioz Stars, 2026-03
90/100 stars
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bisulphite pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc bisulphite pyrosequencing
Example of how some gene regions were chosen for examination in this study on the basis of available RRBS <t>DNA</t> <t>methylation</t> profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE
Bisulphite Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bisulphite pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
bisulphite pyrosequencing - by Bioz Stars, 2026-03
90/100 stars
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quantitative bisulfite pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc quantitative bisulfite pyrosequencing
Example of how some gene regions were chosen for examination in this study on the basis of available RRBS <t>DNA</t> <t>methylation</t> profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE
Quantitative Bisulfite Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantitative bisulfite pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
quantitative bisulfite pyrosequencing - by Bioz Stars, 2026-03
90/100 stars
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psq 96hs machine  (BIOTAGE)
90
BIOTAGE psq 96hs machine
Example of how some gene regions were chosen for examination in this study on the basis of available RRBS <t>DNA</t> <t>methylation</t> profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE
Psq 96hs Machine, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psq 96hs machine/product/BIOTAGE
Average 90 stars, based on 1 article reviews
psq 96hs machine - by Bioz Stars, 2026-03
90/100 stars
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psq hs96gold snp reagents  (BIOTAGE)
90
BIOTAGE psq hs96gold snp reagents
Example of how some gene regions were chosen for examination in this study on the basis of available RRBS <t>DNA</t> <t>methylation</t> profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE
Psq Hs96gold Snp Reagents, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psq hs96gold snp reagents/product/BIOTAGE
Average 90 stars, based on 1 article reviews
psq hs96gold snp reagents - by Bioz Stars, 2026-03
90/100 stars
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line1 bisulfite pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc line1 bisulfite pyrosequencing
Example of how some gene regions were chosen for examination in this study on the basis of available RRBS <t>DNA</t> <t>methylation</t> profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE
Line1 Bisulfite Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/line1 bisulfite pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
line1 bisulfite pyrosequencing - by Bioz Stars, 2026-03
90/100 stars
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illumina humanmethylation450  (Illumina Inc)
90
Illumina Inc illumina humanmethylation450
Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction <t>mammoplasty)</t> from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats
Illumina Humanmethylation450, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illumina humanmethylation450/product/Illumina Inc
Average 90 stars, based on 1 article reviews
illumina humanmethylation450 - by Bioz Stars, 2026-03
90/100 stars
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pyrosequencing pyros  (Pyrosequencing Inc)
90
Pyrosequencing Inc pyrosequencing pyros
Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction <t>mammoplasty)</t> from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats
Pyrosequencing Pyros, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pyrosequencing pyros/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
pyrosequencing pyros - by Bioz Stars, 2026-03
90/100 stars
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methylation analysis (pma)  (Pyrosequencing Inc)
90
Pyrosequencing Inc methylation analysis (pma)
Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction <t>mammoplasty)</t> from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats
Methylation Analysis (Pma), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/methylation analysis (pma)/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
methylation analysis (pma) - by Bioz Stars, 2026-03
90/100 stars
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450 k arrays  (Illumina Inc)
90
Illumina Inc 450 k arrays
Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction <t>mammoplasty)</t> from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats
450 K Arrays, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/450 k arrays/product/Illumina Inc
Average 90 stars, based on 1 article reviews
450 k arrays - by Bioz Stars, 2026-03
90/100 stars
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epitect bisulfite kit  (Qiagen)
90
Qiagen epitect bisulfite kit
Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction <t>mammoplasty)</t> from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats
Epitect Bisulfite Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epitect bisulfite kit/product/Qiagen
Average 90 stars, based on 1 article reviews
epitect bisulfite kit - by Bioz Stars, 2026-03
90/100 stars
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pyromark q96 id system  (Qiagen)
90
Qiagen pyromark q96 id system
Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction <t>mammoplasty)</t> from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats
Pyromark Q96 Id System, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pyromark q96 id system/product/Qiagen
Average 90 stars, based on 1 article reviews
pyromark q96 id system - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Example of how some gene regions were chosen for examination in this study on the basis of available RRBS DNA methylation profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE

Journal: BMC Cancer

Article Title: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation

doi: 10.1186/s12885-015-1777-9

Figure Lengend Snippet: Example of how some gene regions were chosen for examination in this study on the basis of available RRBS DNA methylation profiles for breast cancer cell lines and normal cell cultures and tissues visualized in the UCSC Genome Browser . a The EN1 gene structure with exons as heavy horizontal bars; b , the aligned CpG islands in the illustrated region.; c , DNA methylation (ENCODE/RRBS/HudsonAlpha) profiles for the indicated cell cultures and normal tissues using an 11-color, semi-continuous scale (see color key) to indicate the average DNA methylation levels at each monitored CpG site; d , aligned transcription results indicating that the non-transformed breast cancer cell line is not transcribing this gene irrespective of its lack of DNA methylation. Paradoxically, normal myoblasts are transcribing it despite some upstream DNA methylation. All data are from ENCODE

Article Snippet: Using a candidate gene approach on a large, ethnically diverse set of subjects, we compared not only invasive breast cancer and adjacent histologically normal tissue (as in the TCGA Illumina HumanMethylation450 database [ ]), but also control samples of reductive mammoplasty tissue from non-cancer patients using a quantitative, gold-standard method for DNA methylation analysis (bisulfite/pyrosequencing) amenable to archival FFPE samples.

Techniques: DNA Methylation Assay, Transformation Assay

Mean percent methylation by gene and tissue type from the Breast Cancer Care in Chicago study

Journal: BMC Cancer

Article Title: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation

doi: 10.1186/s12885-015-1777-9

Figure Lengend Snippet: Mean percent methylation by gene and tissue type from the Breast Cancer Care in Chicago study

Article Snippet: Using a candidate gene approach on a large, ethnically diverse set of subjects, we compared not only invasive breast cancer and adjacent histologically normal tissue (as in the TCGA Illumina HumanMethylation450 database [ ]), but also control samples of reductive mammoplasty tissue from non-cancer patients using a quantitative, gold-standard method for DNA methylation analysis (bisulfite/pyrosequencing) amenable to archival FFPE samples.

Techniques: Methylation, Control

Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction mammoplasty) from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats

Journal: BMC Cancer

Article Title: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation

doi: 10.1186/s12885-015-1777-9

Figure Lengend Snippet: Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction mammoplasty) from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats

Article Snippet: Using a candidate gene approach on a large, ethnically diverse set of subjects, we compared not only invasive breast cancer and adjacent histologically normal tissue (as in the TCGA Illumina HumanMethylation450 database [ ]), but also control samples of reductive mammoplasty tissue from non-cancer patients using a quantitative, gold-standard method for DNA methylation analysis (bisulfite/pyrosequencing) amenable to archival FFPE samples.

Techniques: Methylation, DNA Methylation Assay, Control

Adjusted differences in mean % methylation comparing adjacent (referent) to cancer tissue, overall and stratified by ER/PR status

Journal: BMC Cancer

Article Title: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation

doi: 10.1186/s12885-015-1777-9

Figure Lengend Snippet: Adjusted differences in mean % methylation comparing adjacent (referent) to cancer tissue, overall and stratified by ER/PR status

Article Snippet: Using a candidate gene approach on a large, ethnically diverse set of subjects, we compared not only invasive breast cancer and adjacent histologically normal tissue (as in the TCGA Illumina HumanMethylation450 database [ ]), but also control samples of reductive mammoplasty tissue from non-cancer patients using a quantitative, gold-standard method for DNA methylation analysis (bisulfite/pyrosequencing) amenable to archival FFPE samples.

Techniques: Methylation

Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction mammoplasty) from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats

Journal: BMC Cancer

Article Title: Exploring DNA methylation changes in promoter, intragenic, and intergenic regions as early and late events in breast cancer formation

doi: 10.1186/s12885-015-1777-9

Figure Lengend Snippet: Mean percent methylation and 95 % error bars by gene and tissue type for the DNA regions listed in Table . a DNA methylation analysis of samples from the Breast Cancer Care in Chicago study (2005-2008) as determined by our bisulfite pyrosequencing. Control samples (reduction mammoplasty) from unaffected women are represented by green bars, cancer-adjacent, histologically normal samples by blue bars and cancer samples by red bars. b Bioinformatic analysis of DNA methylation of breast cancer samples and paired non-cancerous adjacent samples from The Cancer Genome Atlas (TCGA). Paired non-cancerous adjacent samples are represented by blue bars and cancer samples by red bars. In both panels, promoter sequences are displayed first, followed by upstream sequences, then introns and lastly, DNA repeats

Article Snippet: Using a candidate gene approach on a large, ethnically diverse set of subjects, we compared not only invasive breast cancer and adjacent histologically normal tissue (as in the TCGA Illumina HumanMethylation450 database [ ]), but also control samples of reductive mammoplasty tissue from non-cancer patients using a quantitative, gold-standard method for DNA methylation analysis (bisulfite/pyrosequencing) amenable to archival FFPE samples.

Techniques: Methylation, DNA Methylation Assay